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At the time of the assay, the medium was removed, and cells were washed twice with DMEM without phenol red
And if your health history puts you at high risk (thyroid cancer, pancreatitis, pregnancy), GLP-1s are not for you
These agents have a beneficial effect on pancreatic islet function by facilitating the secretion of insulin in response to ambient blood glucose levels and inhibiting glucagon secretion both directly and indirectly
However, some patients do experience thirst while taking these medications, typically as an indirect consequence of gastrointestinal side effects such as nausea, vomiting, or diarrhea, which can lead to dehydration
10.1101/2021.02.12.430931 bioRxiv 5 AnG.WeiB.XiaB.McDanielJ