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Nature Medicine , 25(7), 1231-1241
When ambient temperature exceeds 8C, two degradation pathways accelerate: peptide bond hydrolysis (breaking the glycyl-histidyl linkage) and copper ion dissociation (loss of the Cu from the peptide backbone)
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2.12.4 Quenching of fluorescence A very promising spectroscopic technique for evaluation of solvent accessibility of aromatic residues in a protein involves the quenching of their intrinsic fluorescence by the addition of various low molecular weight agents (Lehrer, 1971