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The (His) 6 -tag was removed by overnight thrombin cleavage, followed by polishing with size-exclusion chromatography (SEC) using a Superdex 75 HiLoad 16/600 column (Cytiva, MA, USA) equilibrated in 20 mM Tris-HCl pH 8.0, and 150 mM NaCl

This is akin to the post-hoc analysis of tildrakizumab (anti-IL-23) which showed comparable efficacy in patients with and without metabolic syndrome

Overall, these results indicate a possible beneficial role of the microbial shifts induced by metformin for the improvement of the glycemic and metabolic status in T2D

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At the same time, iron-dependent lipoxygenase (LOX) catalyzes polyunsaturated fatty acids (PUFAs) to induce OS, which further alters the permeability of BBB (Figure 2)

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