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Similarly, after approximately 20 g/L of soluble sugars was consumed in the medium containing 4% (v/v) molasses and 5% (v/v) CSL, the feeding solution composed of 50% (v/v) molasses and 25% (v/v) CSL was introduced at 3.513.6 mL/h

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All signals were visualized signal by adding the chemiluminescent ECL substrate (Boster Biological Technology, CA, USA) and exposure to X-ray film