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Following the digestion of the genomic DNA by DNase I (Thermo Scientific, Waltham, MA, USA), samples were cleaned and concentrated using RNA Clean Concentrator-25 Kit (Zymo Research, Irvine, USA)

There are multiple excellent reviews on medical approaches to disease modification in PD and appropriate clinical trial formats, but these have generally excluded surgical treatments 9,11 , whereas individual reviews of gene therapy, regenerative medicine strategies, and stem cell transplantation in PD 12,13 do not critically discuss comparative potential efficacy and disease modification across surgical approaches 10

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