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Protoplast Isolation and Transfection Two-week-old seedlings grown on 1/2 MS were used to isolate protoplasts as described previously with minor modifications ( Transcriptome Analysis Total RNA was extracted from 3-week-old plants with TRIzol (Invitrogen) and purified using a GeneJET plant RNA purification kit (Thermo Fisher Scientific)

1 2 J Int Med Res

The biggest mistake people make is trying multiple remedies at once