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Beyond weight loss: Comparative effects of tirzepatide plus low-energy ketogenic versus low-calorie diet on hepatic steatosis and stiffness in MASLD

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All assays were run as per manufacturers recommendation, an overnight supernatant incubation protocol was used for all assays except Angiogenesis Panel 1 and Vascular Injury Panel 2, which were run according to the same day protocol

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Results revealed that muscle stem cell proliferation and fusion were substantially elevated in treated aged mice, supporting nearly twofold greater myofiber cross-sectional area and shifts in fiber size distribution toward larger, healthier muscle fibers