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In addition to GPX4 and NRF2, cells employ various antioxidant mechanisms, including SOD, catalase (CAT), and vitamins C and E, to combat oxidative stress (Chen et al., 2016)

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When it binds with copper (Cu), it activates enzymes responsible for: connective tissue formation antioxidant defense cellular respiration It also regulates free copper ions to prevent oxidative stress, keeping the system balanced rather than reactive

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