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Western blotting followed by enhanced chemiluminescence detection was performed as previously described 30
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[DOI] [PubMed] [Google Scholar] 49.Aronne L.J., Sattar N., Horn D.B., et al
Concentrations decline sharply with age: plasma levels drop from roughly 200 ng/mL at age 20 to under 80 ng/mL by age 60
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