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Cell culture and imaging HeLa cells were cultured in minimum essential medium containing 10% fetal bovine serum and antibiotics (100 units/mL penicillin and 100 g/mL streptomycin) in a 5% CO 2 humidity incubator at 37C

The thyroid gland will try to overcompensate and produce more thyroid hormones (T3 and T4) to keep the levels normal even as the gland and the hormones themselves are being destroyed