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We separated nuclear and cytoplasmic proteins and calculated the ratio of p-P53 to total P53 in each fraction

[1] Catalytic mechanism of phosphopentose isomerase The catalytic mechanism of phosphopentose isomerase resembles that of phosphohexose isomerase (EC 5.3.1.9), a glycolytic enzyme

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10.1128/iai.61.7.2848-2853.1993 94 KapurN.GrimwoodK.MastersI

Following this process, 0.5 g of DTT was mixed with 3 ml of as-prepared carbon dots solution and incubated overnight with continuous stirring at 500 rpm to obtain thiol functionalized quantum dots