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Once outside the cell, GSH degradation occurs exclusively in the extracellular space and only on the surface of cells that express the ectoenzyme -glutamyl transferase

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This solvent was composed of 40 L of 50 mM NEM (Sigma-Aldrich) in a mixture of 80% methanol and 20% 10 mM ammonium formate (Sigma-Aldrich), combined with 40 L of 100% acetonitrile (Thermo Fisher)

These psychosocial factors can be more influential on mental wellbeing than any direct neurochemical effects of the medication