Once colonies were found, competent cells were prepared and transformed with a URA resistance cassette with homology arms to endogenous CHC1 gene (See Supplementary Table 5 for primer S2/S3 sequence) using the established PCR cassette protocol (pFA6a-KlURA3 Vector) 65
The biggest slam dunk outcome is triglyceride reduction , while other outcomes depend on dose, population, and baseline diet
Amaranth proteins are dominated by albumins (water-soluble proteins) and 11S globulins (storage proteins found in seeds), which represent between 44% and 62% of the total protein (Tandang-Silvas et al., 2012)
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Consistent with this, we found that SYNCRIP facilitates IRES-mediated translation of HK2, suggesting a role for SYNCRIP in glycolytic activity
