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Several studies have reported that directly supplementing TMAO into the diet of mouse and zebrafish models promotes NAFLD [14, 85]

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The abdomen is often preferred by patients and healthcare providers due to its accessibility, consistent subcutaneous fat layer, and ease of self-administration

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After washing with PBS, cells were incubated in 2 mM Ca 2+ buffer containing 5.5 or 16.7 mM glucose, 0.1% BSA, and 10 nM GLP-1 or GIP for 60 min at 37C