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Methods Materials For tissue culture Dulbeccos modified Eagles medium (DMEM), RPMI-1640 medium, hygromycin-B, and Fluo-4 acetoxymethyl ester were used (Invitrogen Carlsbad, CA)

Results may differ for earlier-stage patients Combined treatment effect: Both groups received radiofrequency, so the study measures the added effect of the serum not the serum alone

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