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Step 3 Make sure the needle is placed at a 90 degree angle to the skin

48 to 72 hours B12 injections work quickly and typically show noticeable effects within 48 to 72 hours

Last reviewed: June 9, 2026 Last updated: June 9, 2026 Written by: Jay Hastings, CEO of PlexusDx Jay Hastings is the CEO of PlexusDx, a precision health company focused on genetic testing, blood biomarker insights, and personalized wellness recommendations

Disclaimer: This article is intended for licensed medical professionals

B12 facilitates the breakdown of protein and fat so that your body can utilize them

For those without an absorption problem, research suggests that high-dose oral B12 supplements can be as effective as injections in raising and maintaining B12 levels