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Tissue was gently teased apart in BIOPS with forceps on ice, followed by permeabilization with saponin (50 g/mL) for 30 min on a rocker at 4 C

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Oral GLP-1 programs remained minimal until the late 2000s, then began rising meaningfully post-2018with the most visible step-up in the last few years: 10 oral programs in 2022, and 11 each in 2024 and 2025