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Western blot analysis TSPCs were cultured in medium alone (N group) or with 500 M NAC (NAC group) in the presence or absence of LY294002 (Bioss), an inhibitor of the phosphoinositide 3-kinase (PI3K)/AKT signaling, as the intervention group for 1 and 2 weeks (the same medium for each group of cells was replaced every three days)

Consequently, the observed reduction in MDA levels following AOX therapy highlights its therapeutic significance in alleviating OS and improving outcomes in OS-related male infertility