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The samples were incubated for 1 h at RT, washed three times with TBST, reacted with Supersignal West Pico solution (Pierce) for 5 min, and exposed to X-ray film (Agfa)

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The large majority of published studies were carried out with only a limited number of patients, and to date, no large-scale, double blind randomized trials that are imperative in determining the clinical efficacy of IVC have been completed