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To obtain a GLP-1RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag

CJC-1295 acts on the GH axis, not the HPG (hypothalamic-pituitary-gonadal) axis