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[2] High-dose intramuscular administration creates a tissue depot that releases vitamin D slowly over weeks, potentially enabling a single injection to lift serum 25-hydroxyvitamin D levels into the sufficient range in severe deficiency and to potentially support bone mineralisation, neuromuscular function, and secondary hyperparathyroidism reversal while bypassing the gastrointestinal tract

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Later work examined NNMT inhibition combined with dietary changes in obese mice, reporting reductions in body weight, fat mass, and hepatic fat, alongside shifts in gut bacterial populations

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