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Islet isolation and glucose-stimulated insulin secretion Pancreatic islets were isolated through collagenase digestion of the pancreas, followed by gradient purification to obtain intact islets

Uptake by BMECs after IR-EM-binding peptide (IEP) blockade BMECs were preseeded in 12-well plates (2 10 5 per well) and cultured at 37 C for 24 h, after which the medium was discarded, and fresh medium added that contained IEP at final IEP concentrations of 0.8%, 0.4%, and 0.2% (molar ratio) of the phospholipids, and the cells were then incubated for 1 h

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Evolving insights into the pathophysiology of diabetic neuropathy: implications of malfunctioning glia and discovery of novel therapeutic targets