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5ad) by 2 folds, based on the in-gel fluorescence (Fig

Lentivirus was produced by transfecting HEK293T cells plated in a T75 flask with 6 g of psPAX2, 3 g of pMD2.G and 12 g of lentiviral vector containing the LaccID, TCR or CAR with 60 l of PEI Max (1 mg ml 1 in water, pH 7.3)

J Neurosci 23:508895, PMID: 12832532 Dallas M, Boycott HE, Atkinson L, Miller A, Boyle JP, Pearson HA, Peers C (2007) Hypoxia suppresses glutamate transport in astrocytes

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Ive used BPC probably 20 different times, he says