It therefore seems that GLP-1/exendin-4 might be acting in the VMH and LH, interacting with the AMPK/S6K signalling pathways, and modulating the activation status of AMPK and S6K in response to nutrient fluctuations
The role of neuropeptides in learning: focus on the neurokinin substance P
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Cells were harvested after 24 h in PBS and crosslinked using 1% formaldehyde at room temperature for 7 min, followed by 3 min centrifugation at 1800 g