Onset: Localized respiratory effects are often noticed within the session
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2.5 Quantification of oxidative phosphorylation and glycolysis The Seahorse XF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, United States) was utilized to measure real-time integrated cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) as previously outlined ( 2.6 Western blotting (WB), real-time quantitative PCR (RT-qPCR) and reactive oxygen species (ROS) analysis WB analysis, RT-qPCR, and ROS analysis were performed as previously described (Supplementary Table S1
Mitochondria are the cells power plants
This is partly because the drugs effects occur so fast, and the number of mechanisms that too much of the drug in the system can disable
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