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PLoS One 9:e109767

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[DOI] [PMC free article] [PubMed] [Google Scholar] 28.Yu M., Li H., Liu Q., Liu F., Tang L., Li C., Yuan Y., Zhan Y., Xu W., Li W., et al

Immunofluorescence staining Primary murine chondrocytes and RAW 264.7 murine macrophage cell line were fixed with 3.7% PFA for 30 min at RT, permeabilized with TBS + 0.05% Tween 20 buffer for 30 min at RT, and blocked with 2.5% normal horse serum for 20 min at RT