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Different research approaches addressed

DOI: 10.1016/S1474-4422(06)70628-7 17

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First, a bacterial reverse mutation test (Ames test) assessed AOD9604s mutagenic potential using four histidine-dependent auxotrophic mutants of Salmonella typhimurium (TA1535, TA1537, TA98, TA100) and one tryptophan-dependent auxotrophic mutant of Escherichia coli (WP2 uvrA), using both plate incorporation and pre-incubation methods at doses of 0, 1.6, 8, 40, 200, 1,000, and 2,000 g AOD9604/plate

8 weeks (10 vials): 30 mL 3 10 mL bottles 12 weeks (19 vials): 57 mL 6 10 mL bottles 16 weeks (31 vials): 93 mL 10 10 mL bottles Alcohol Swabs: One for the vial stopper + one for the injection site each day

This effect plays a critical role in maintaining intestinal homeostasis