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In addition, our previous studies revealed that microglia and their purinergic receptors (P2 4R, P2 7R and P2Y12R) were significantly upregulated in the TNC after NTG injection, and inhibiting microglial activation, including morphological and inflammatory changes, might affect neuronal hyperexcitability in the TNC, which ultimately relieved CM-associated allodynia [17,18,19]

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Following that, electroporation, a well-established technique in the field of biomedical research, entails the controlled administration of brief electric pulses to the skin, resulting in the transient formation of pores [10]