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Running water down the vial wall instead of directly onto the powder is a small procedural detail that makes a real difference

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Procedure : Initially an experiment was performed that demonstrated the presence of glutathione in the liver cytosol fraction

After washing once with Annexin-V binding buffer, cells were acquired via flow cytometry (LSRII, BD Bioscience, Heidelberg, Germany) and data were analyzed with FlowJo X (FlowJo LLC, Ashland, OR, USA)

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