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The OCR was measured in real-time, quantifying the picomoles of oxygen consumed per minute (pmol/min), and graphed over time, before and after the addition of Rotenone to the culture medium (baseline OCR and specifically used for measuring mitochondrial respiration)

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SGLT2i, sodium-glucose cotransporter-2 inhibitor

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Multiple epigenetic clocks were evaluated: first-generation (Horvath, Hannum), second-generation (PhenoAge, GrimAge, including principal-component GrimAge (PCGrimAge), a mortality-linked estimator), the third-generation DunedinPACE, which indexes the pace of aging, and OMICmAge (multi-omic methylation age)

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