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For characterization of cARLA-treated hECs the cells were cultured in 50% PC-conditioned medium on glass bottom chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific) coated with collagen IV and fibronectin at a seeding density of 5 10 4 cells/cm 2
It facilitates continuous interaction, capacity-building, and the sharing of knowledge among CoP members, regardless of their geographical location
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This approach protects immune function and supports safe outcomes
Additionally, in most cases samples are not pre-treated with a masking agent or the agent is added after acid addition, all conditions where measured basal levels result to be very high
