Under physiological conditions, more than 98% of the total GSH pool exists in the reduced form, thereby shaping cellular redox potential and influencing proliferation, differentiation, and cell death 5 .Although the GSH/GSSG ratio has long been regarded as an indicator of cellular redox status, recent live-cell studies using genetically encoded redox sensors indicate that increases in GSSG are typically transient and compartmentalized rather than sustained intracellular accumulations 6,7
This research-grade peptide is intended for laboratories and trained research professionals
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Quantitative RT-PCR RNA was isolated using a NucleoSpin kit (Machery-Nagel, 740955.50) and converted in cDNA using an iScript cDNA Synthesis Kit (Biorad)
Sorbitol, unlike glucose, cannot pass efficiently through cell membranes and therefore remains trapped inside the cells, increasing osmolality, resulting in swollen cells due to water retention
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