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They concluded that while GHK-Cu does demonstrate anti-wrinkle effects in cellular studies, "there is a surprising absence of clinical studies using them." The review identified skin permeation as the central challenge: GHK's high aqueous solubility and low partition coefficient work against transdermal absorption

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Funding We work in this area has been supported by the National Health and Medical Research Council (NHMRC) of Australia (ID: APP1147333), the National Natural Science Foundation of China (NSFC, ID: 81870561), Royal Adelaide Hospital Research Foundation, and the University of Adelaide

Maintaining sterility during injection is important to avoid infections