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Buthionine sulfoximine (BSO), 1, 3-bis (2-chloroethyl)-1-nitrosourea (BCNU), diamide, and Hoechst 33342 were purchased from Sigma (St

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containing 1 mM EDTA), after which, the sample was centrifuged at 10,000 x g for 15 min at 4C and the supernatant was removed