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Namely, oxidative stress markers, monoamine levels, electrocorticography, pain threshold test, and open field test were conducted on control, FM-induced, and FM-treated groups of animals

This ratio was vehicle-corrected by subtracting the response of vehicle-treated wells from the ratio of ligand-treated wells and then baseline-corrected by subtracting to the mean BRET ratio of baseline values (prestimulation) for each well