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Effect of sperm cryopreservation in farm animals using nanotechnology

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Bojanowski K, Ibeji CU, Singh P, Swindell WR, Chaudhuri RK

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Cells were seeded in complete medium consisting of RPMI-1640 supplemented with 10% FCS (Sigma), 1% Penicillin/Streptomycin (GIBCO), 1% L-Glutamine (Sigma), and 55 mM -mercaptoethanol (GIBCO)