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43 The Semaglutide Treatment Effect in People with obesity (STEP) Program trials, with semaglutide 2.4 mg and lifestyle intervention, showed weight reductions of 14.717.4% on average in persons with pre-obesity or obesity (without diabetes), with improvements in cardiometabolic risk factors and sustained effects for two years (Table 2)

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The absorbance at 470 nm was measured for 10 min with readings every minute