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If one method was used in more than one article, their results were entered in the Revman5.4 software and were meta-analyzed

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The tissue was lysed on ice for 30 min, then triturated with a tissue homogenizer and centrifuged to obtain the supernatant, which was called tissue protein sample

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Molecular docking The energy-minimum conformation of hydroxyurea for docking was obtained by the conformation search function of Molecular Operating Environment (MOE v2019.0102, Chemical Computing Group ULC, Montreal, QC, Canada)

The landmark STEP trial compared semaglutide plus lifestyle changes to lifestyle changes alone