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Immunoblot analysis Whole cell lysates were prepared using radio-immunoprecipitation assay (RIPA) lysis buffer (pH 7.8) containing 25 mM Tris-HCl, 120 mM NaCl, 5 mM EDTA and 0.5% NP-40 supplemented with 100 g/mL phenylcmethylsulfonyl fluoride, 200 mM sodium orthovanadate, 0.5 M NaF, protease inhibitor cocktail sets III and IV and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

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Collagen supplements have been tested in many studies to reduce pain and/or improve function in osteoarthritic knees

In controlled laboratory conditions, it may be included in experimental studies examining peptide stability, metal coordination, and molecular interaction behavior

Vitamin B12 is an essential, water-soluble nutrient for vitality and wellbeing

A new labdane diterpene from the rhizomes of Alpinia officinarum