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Tyrosine hydroxylase is involved in the synthesis of catecholamines, leading to heightened production of substances like dihydroxyphenylalanine (DOPA), norepinephrine, and dihydroxyphenylglycol (DHPG) (Buffington et al., 2002)

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Regarding U-87MG, a clear separation of the exometabolome was observed in the PCA analysis comparing U-87MG cells transfected with the anti- GLS1 siRNA and the controls (control and lipofectamine) (Fig