After the experiment, spikes were sorted using Off-line Sorter software (Plexon Inc.)
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Our fitting template included 17 metabolites (alanine, aspartate, choline, creatine, GABA, glucose, glutamate, glutamine, GSH, glycine, lactate, myo-inositol, N-acetyl aspartate, N-acetyl aspartyl glutamate, phosphorylethanolamine, scyllo-inositol, and taurine)
3 The ratio of cyan pixel intensity to the total number of pixels in the biopsy region, multiplied by 1,000, was used to calculate the SA-gal values, which were then reported as arbitrary units (AU)