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Such phosphorylation might affect proteins like voltage-dependent calcium channels on pituitary cell membranes, possibly resulting in channel opening, calcium influx, and subsequent release of growth hormone from secretory vesicles

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2.11 Separation and extraction of nuclear and cytoplasmic proteins In this experiment, nuclear and cytoplasmic fractionation was performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0027) following the manufacturers instructions