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Ankylosing Spondylitis Axial Spondyloarthritis, 3rd ed

Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

Fluorescent images were acquired using a fluorescence microscope, and data were quantified with ImageJ software

Acta Biol Hung 66:258269 Goraca A, Piechota A, Huk-Kolega H (2009) Effect of alpha-lipoic acid on LPS-induced oxidative stress in the heart