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If you must, aliquot the solution into single-use portions and freeze them once, thawing only what you need immediately before use

RNA-seq and data analysis Activated murine ILC2s were cultured for 24h Melatonin (250M)/DMSO, lysed in RLT buffer (Qiagen), and RNA was extracted using the MicroRNeasy kit (Qiagen)

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doi: 10.1038/nrd.2016.248 27 SunYXiaXBasnetDZhengJCHuangJLiuJ

Mead RJ, Higginbottom A, Allen SP, Kirby J, Bennett E, Barber SC, et al