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In-vitro studies have reported that GHK-Cu can decrease the expression and activity of certain MMPs (like MMP-1 and MMP-2) while increasing the expression of TIMPs (TIMP-1, TIMP-2)

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To achieve this, cells were fixed with 4% paraformaldehyde (P0099-100ml, Beyotime, Shanghai, China), permeabilized, stained with 0.1% crystal violet (G1064, Solarbio, Beijing, China), and measured using a plate reader (Molecular Devices) through spectrophotometry after the cells were dissolved with 10% acetic acid (A298827-500ml, Aladdin, Shanghai, China)

Others take 13 days

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