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The hybridization buffer contains: 5xSSC, 50% deionized formamide, 100 g/ml autoclaved Herring sperm DNA, 50 g/ml Heparin and 0.1% Tween-20

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This potency was lost when GHS was given to lab animals with hypophyseal stalk section or to human subjects suffering hypothalamic pituitary disconnection as the result of disease or trauma 4