The -cyano amide-based Michael acceptors have also been elegantly used by the Taunton group as warheads in small-molecule inhibitors to react with active site cysteines while being minimally affected by the high concentration of intracellular GSH due to the fast reaction kinetics and the mM of K d (refs 31, 32)
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Clinically overt HT affects approximately 0.12% of the population, while the subclinical form is more prevalent, affecting 1015%
