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Adaptors were trimmed from de-multiplexed FASTQ files using Cutadapt ( aligned to the Gecko V1 design file, and count tables were generated with MAGeCK software V5

10.1016/j.cmet.2018.03.008 Cell Metab

Apart from the iron serving as co-factors and the iron stored in ferritin, the remaining divalent iron in the cytoplasm (a concentration of approximately 0.55 m) is called the free iron pool (Espsito et al., 2002