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Abstract Excess fat accumulation has been observed widely in farmed fish

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Incorporating in vivo pharmacokinetic data or clinical evidence would enhance scientific rigor

Science 371 , 11391142 (2021)

After loading cells with calcium dye, counter-staining with Hoechst, and adding synaptic blockers as described above, cultures were imaged at 37.5C and 5% CO in confocal mode with a 20 water immersion objective and 2x binning