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The tubes were centrifuged at 1000 g for 5 min and the supernatant was discarded

Intriguingly however, in glr1 cells, we observed almost no detectable NADP oxidation at all, except for a slight response of the NAPstar3 probe to the highest diamide concentration (5 mM) ( Figure 5C,E )

For a subcutaneous injection, the needle is inserted at a 45-degree angle so that it pierces the skin but does not reach the muscle tissue